Study of Angiogenesis after the Experimental Acute Cholecystitis

The experimental study aimed to induce the inflammation of the cholecyst in 40 pigs, injecting L-α-Phosphatidylcholine in the gallbladder using laparoscopy and to establish the presence of angiogenesis in the healing process. Some groups received then Dicolfenacum and some groups did not, until the laparoscopic cholecystectomy, which was performed after 3 or 7 days. Hematoxylin and eosin stains were done from the gallbladder sections. At the reintervention moment there were noticed many adherences. The histology emphasized the gallbladder’s inflammation, colagenolysis and vascular alteration. When the action of L-α-Phosphatidylcholine decreases (or disappears), vascular repair processes are very active and the edema disappeared, making it safe to start the repair processes. In the area where repair processes are well represented, many blood vessels are present, they are neoformation vessels. Histopathological examination proved that at 3 days L-αPhosphatidylcholine produced a severe inflammation of the gallbladder’s wall and comprised all of his structures from the surface to depth with marked vascular changes and recent thrombus in some small vessels. At 7 days after administration of L-αPhosphatidylcholine histopathological examinations show in mid-depth repair processes presenting active fibroblasts, thin collagen fibers and neoformation vessels.